Background: The main aims of the study were to explore the frequency of missed nursing care (MNC) among Czech hospital nurses, and to find the relationship between nurse’s job satisfaction and MNC. Material and Methods: The sample consisted of 513 nurses from 9 hospitals in the Czech Republic. Data were collected in January–August 2019 using the standardized MISSCARE Survey questionnaire, complemented with items assessing nurse’s job satisfaction and demographic data. Results: The most frequently missed nursing activities were patient ambulation and emotional support to the patient and/or family. The surveyed nurses were most satisfied with being a nurse and least satisfied with the level of teamwork on their unit. The strongest correlation was found between satisfaction with the current position and satisfaction with being a nurse; there was a negative correlation between satisfaction with the current position and the overall level of MNC. There was a significant trend between the rating of satisfaction with the current position and MNC. Conclusions: Nurse’s job satisfaction is associated with the level of nursing care provided; more missed care means more dissatisfaction among nurses. Med Pr. 2021;72(3):231–7