Background: The aim of the study was to examine whether gain or loss of personal resources is associated with conflict or facilitation between work and family. Material and Methods: The study involved 90 employees (mean age: 34.5 years). The Polish version of COR-Evaluation (Conservation of Resources-Evaluation) questionnaire, developed by Hobfoll and adapted by Dudek et al, was used to assess personal resources. The questionnaire enables to estimate gain and loss of 40 resources and calculate the overall level of gained or lost resources. SWING Questionnaire (Survey Work-Home Interaction, Nijmegen), developed by Geurts et al. and adapted by Mościcka-Teske and Merecz), was used to examine the quality of work–home and home–work relationship. Results: The gain of personal resources positively correlates with both home–work facilitation (HWF) and work–home facilitation (WHF). Improvement of the family relations proved to be the most significant predictor of HWF and WHF. The loss of personal resources coincides with high level of conflict between the investigated areas of life. The main predictor of home–work conflict (HWC) was the variable relating to restrictions of access to medical services. In case of work–home conflict (WHC) the reduction of material security in case of dramatic life events was the major predictor. Conclusions: The results confirmed that the gain of resources is crucial for HWF/WHF, while their loss is an important factor, when the HWC/WHC is considered. The resources, which proved to be the main predictors of work–home and home–work relationship were also indicated. The obtained information may be beneficial to human resources managers, especially in designing the activities aimed at increasing the satisfaction and effectiveness of employees. Med Pr 2014;65(6):755–764