The influence of methionine and vitamin E on oxidative stress in rats’ liver exposed to sodium fluoride
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Śląski Uniwersytet Medyczny w Katowicach / Medical University of Silesia in Katowice, Zabrze, Poland (Wydział Lekarski z Oddziałem Lekarsko-Dentystycznym w Zabrzu, Katedra Biochemii / School of Medicine with the Division of Dentistry in Zabrze, Department of Biochemistry)
Śląski Uniwersytet Medyczny w Katowicach / Medical University of Silesia in Katowice, Sosnowiec, Poland (Wydział Farmaceutyczny z Oddziałem Medycyny Laboratoryjnej w Sosnowcu, Zakład Statystyki, Katedra Analizy Instrumentalnej / / School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Department of Statistics, Department of Instrumental Analysis)
Urszula Błaszczyk   

Śląski Uniwersytet Medyczny, Wydział Lekarski z Oddziałem Lekarsko-Dentystycznym w Zabrzu, Katedra Biochemii, ul. Jordana 19, 41-808 Zabrze
Online publication date: 2018-07-23
Med Pr 2018;69(4):403–412
Background: Fluorine influences many processes occurring in the organism. Controversies over the evaluation of the biological effects of this substance are due to a small difference between tolerable and toxic fluorine doses. One of the main mechanisms of the fluorine toxic action is its ability to induce oxidative stress via reactive oxygen species generation and antioxidant defense system impairment. It is important to evaluate possible interactions between fluorine and other substances that may increase or decrease its toxicity. Material and Methods: The study lasted for 35 days. Twenty-four rats were divided into 4 groups: the control, with sodium fluoride (NaF) in the diet, with sodium fluoride, methionine and vitamin E (NaF+M+E) in the diet, with sodium fluoride and vitamin E (NaF+E) in the diet. The biochemical analysis conducted in animal liver homogenates included determination of activities of: total superoxide dismutase (t-SOD), superoxide dismutase with copper and zinc (CuZnSOD), superoxide dismutase with manganese (MnSOD), glutathione peroxidase (GPX), catalase (CAT), glutathione reductase (GR), glutathione S-transferase (GST) and the malondialdehyde (MDA) concentration. Results: The activities of CuZn- SOD, GPX, CAT and MDA concentration were changed significantly. There were no differences in the activities of t-SOD, MnSOD, GR and GST among the experiment. Conclusions: In the conducted experiment, the run-out of enzymatic protection of liver by decreasing of the activities of antioxidant enzymes (CAT and GPX) and increasing the MDA concentration in NaF group was observed. The addition of vitamin E and methionine does not significantly stimulate the enzymatic antioxidant system, however, it causes of MDA concentration decreases. Med Pr 2018;69(4):403–412