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ORIGINAL PAPER
 
CC BY-NC 3.0 Polska
 
 

Microbiological air quality in some kindergartens and antibiotic resistance of bacteria of the Staphylococcus spp. genus

Łukasz Kubera 1  ,  
 
1
Uniwersytet Kazimierza Wielkiego / Kazimierz Wielki University, Bydgoszcz, Poland (Wydział Nauk Przyrodniczych, Instytut Biologii Eksperymentalnej, Zakład Mikrobiologii / Faculty of Natural Science, Institute of Experimental Biology, Department of Microbiology)
2
Uniwersytet Kazimierza Wielkiego / Kazimierz Wielki University, Bydgoszcz, Poland (Wydział Nauk Przyrodniczych, Instytut Biologii Eksperymentalnej, Zakład Immunobiologii / Faculty of Natural Science, Institute of Experimental Biology, Department of Immunobiology)
Med Pr 2015;66(1):49–56
KEYWORDS:
ABSTRACT:
Background: Microbiological contamination of the air and the acquisition of the antibiotic resistance by pathogenic bacteria is a growing phenomenon that has a substantial impact on the quality of our health. This problem applies mainly to public areas where we spend a large part of our lives. This study was focused on the microbiological analysis of the air in some kindergartens and antibiotic resistance of bacteria of the Stephylococcus spp. genus. The identification of the isolated mould fungi has been also made. Material and Methods: Air samples were collected from classrooms in the seasonal cycle in the mornings and afternoons using 2 methods, sedimentation and impact. Air samples collected outside the kindergartens served as controls. Air quality assessments were based on the groups of indicator microorganisms, according to Polish standards. The susceptibility of isolated staphylococci was assessed with the disc-diffusion method, using 8 different classes of antibiotics, in line with the recommendations of the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Results: The analyses show that, regardless of the method, the total number of heterothropic bacteria and staphylococci in the air of the analyzed kindergartens exceeded the allowable limits. There was no air pollution with the fungal infection. Based on the antibiogram, it was found that Staphylococcus spp. strains showed the highest sensitivity to chloramphenicol and the lowest to penicillin and gentamicin. Among the fungi moulds of the genus Cladosporium predominated. Conclusions: The results of the analyses highlight the need for regular health checks and further research to help identify biological factors that may significantly affect the quality of health of people living in public spaces. Med Pr 2015;66(1):49–56
CORRESPONDING AUTHOR:
Łukasz Kubera   
Uniwersytet Kazimierza Wielkiego, Wydział Nauk Przyrodniczych, Instytut Biologii Eksperymentalnej, Zakład Mikrobiologii, ul. Chodkiewicza 30, 85-064 Bydgoszcz
eISSN:2353-1339
ISSN:0465-5893