Populations in large workplaces are particularly susceptible to the risk of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. In the following article, an attempt has been made to identify main problems regarding the biological security in large workplaces outside the healthcare sector, while simultaneously pointing out the possible solutions to these problems. In this article, a literature review was performed with regard to publications that have been published within the last 4 months, concerning the issue of risk factors with regard to SARS-CoV-2 infections in large populations. Experts’ opinions and statements released by institutions working in this field were also taken into consideration. On the basis of the available publications, key procedures to protect workers in large workplaces against COVID-19 were established. One of these key factors is infection prevention. It is commonly known that, due to limited possibilities of identifying infected people that are asymptomatic, physical distance in the whole employee population should be kept; personal protective equipment (PPE) should be used and tele-work should be implemented. The results of recent research have shown that, apart from airborne ways of transmission, there also exists a possibility of getting infected by coming into contact with contaminated surfaces and objects. Therefore, frequent disinfection of rooms and work tools is essential. In the current situation, due to the lack of a vaccine, only going through the COVID-19 disease, either in a symptomatic or asymptomatic form, significantly increases the chances of developing natural immunity. The means of preventing SARS-CoV-2 infections include quickly identifying the infected people on the basis of the symptoms they report, keeping physical distance, using PPE, disinfecting rooms and applying proper ventilation. Limiting the number of workers by implementing a remote work pattern is also recommended. Med Pr. 2021;72(1):89–97